Pichia Pastoris

At Rosta Biotech we used metabolic engineering, molecular biology techniques and fermentation feeding strategies to design new yeast host strains. This is a platform of the methylotrophic yeast strain Komagataella phaffii (syn. Pichia pastoris) and vectors for recombinant protein expression which is generated to facilitate production of proteins in industrial scale. This platform also includes the synthetic parts for tunable gene expression in yeasts to generate new yeast strains. We own all the IP rights and secret know-how for our platform technology. We are skilled at solving problems and managing projects. We ensure 100% freedom-to-operate for all our clients and licensees and provide a smooth and high standard project progress.

Competitive Advantages of Rosta Biotech Strains

• High-level protein production (0.5 – 20 g/L)
• Stable co-integration of multiple expression cassettes
• Short and cost-efficient fermentation without complex additives
• Stable and non-allergenic protein production
• Co-expression of different genes in one strain
• Expression vectors w/o resistance marker
• Low levels of host protein impurities upon secretion